Integration, Reproducibility and Analysis
Scipion is an image processing framework to obtain 3D models
of macromolecular complexes using Electron Microscopy.
Scipion will help you to get your processing organized by projects. Differently to Relion, there is no need to launch Scipion from the project folder. It can be launched anywhere and then you can select the project you want to work on. Let’s start by a new project by launching the following command from a terminal:
Sometimes the CTF estimation algorithm may fail to find the rings even if they can be seen by eye. If this is the case, you may help the algorithm to find the rings by clicking on the image with the mouse right-button and choosing Recalculate CTF in the menu that appears.
A graphical interface will help you to correctly identify the CTF. You must provide the first CTF zero and the search range, and then press OK. When you finish, press red Recalculate CTFs button.
When analyzing the resulting class reconstructions, it is extremely useful to also look at them in slices, not only as a thresholded map in for example UCSF Chimera. In the slices view you will get a much better impression of unresolved heterogeneity, which will show up as fuzzy or streaked regions in the slices. Slices also give a good impression of the atness of the solvent region.
When looking at your rendered maps in 3D, e.g. using UCSF Chimera, it is often a good idea to t them all into the best one, as maps may rotate slightly during refinement.
We install our RELION Cryo-EM Stack as well as SCIPION.
We provide 1 year support of Scipion Installation on our Workstatiosn and Servers.